Summary
Type: SPE
Application #: 302330
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection: An aliquot (20µl) of the solution was analysed by HPLC.
Conditions: Column conditioning: with 3 ml methanol
Sample application: The solution was loaded on to the solid phase cartridge.
Column washing: with 3 ml of water
Elution: with 8 ml of 25% methanol
Evaporation: to dryness under reduced pressure and dissolved in 1,0 ml methanol
Authors: Hösch G Wiedenfeld H Dingermann Th Röder E
Source: Phytochemical Analysis Vol 7 284-288 (1996)
Details
Legend: The total recovery of alkaloids decreased with time of application of the soxhlet extraction procedure. While a PA yield of 0,55% was determined in the plant material after a 6 h extraction this dropp